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This diet was provided five times each day (during 40 days).
The same commercial gestating diet was provided daily at 2.0 kg, 2.2 kg and 2.4 kg/day in the first, second and third parity, respectively, in both treatments.
The embryos of B. mori strain (p50) were hatched and reared as previously described [2], and the artificial diet was provided by the Chinese Academy of Agricultural Sciences.
The high fat diet was provided using Rodent CAFÉ™ feeders (OYC International, Inc., MA), and liquid Ensure was provided in a 30-ml bottle with a rodent sip tube (Unifab Co., MI) and liquid intake was measured every day.
Fresh food diet was provided ad libitum along with adult diet (see below) for one week and then fresh food was reduced gradually over the course of the three weeks until birds were on adult diet by approximately day 60.
Interestingly, when the same HF diet was provided for 6 weeks after weaning, only males issued from normally fed dams become overtly obese while those born to HF dams were protected against the obesogenic effect of the HF diet, despite the same defective hypothalamic leptin signaling.
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The difference with the Atkins approach was that 70% of the calories in the diet were provided by fat, compared with the healthy recommended limit of 35% fat calories, which most other commercial slimming programmes easily adhere to.
Many animals generally considered carnivores are actually omnivorous, among them the red fox, which enjoys fruits and berries, and the snapping turtle, one-third of whose diet is provided by plants.
Many fish can be held in captivity and will spawn naturally when proper environment and appropriate diet are provided (Watanabe and Vassallo-Agius 2003).
Enriched vitamin water and a poultry maintenance diet were provided ad libitum except during experimental periods (see below).
All diets were obtained from Harlan Laboratories (Madison, WI) and the detailed composition of each diet is provided as supplementary data (Additional file 1: Table S1).
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