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Two types of hay (aNDFom sources) and two types of starch were each included at two different levels in the diet and were examined in an experiment following a 2×2×2 factorial arrangement of treatments (eight diet combinations).
In P1, each diet was presented solely for five days to facilitate pre- and post-ingestive associations; in P2 a two-choice test was conducted with four diet combinations (contrasts) over three days; and in P3 the four diets were presented simultaneously in a checkerboard fashion over a 5-day period.
Hierarchical clustering analysis clearly highlighted differences in expression of these genes across steer type and diet combinations (Additional File 1).
A comparison of cardiac gene expression between all diet combinations together identified a subset of 111 DEGs significant only in males, 64 DEGs significant in females only, and 74 transcripts identified as differentially expressed in response to dietary manipulation in both sexes.
A final analysis of significant diet-induced deregulation of cardiac gene expression between all diet combinations co-analyzed yielded a cohort of 97 mapable genes which were deregulated by all dietary manipulations only in males, and half that number of genes which were only deregulated in females.
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Pondimin and Redux, once parts of the fen-phen diet combination, were taken off the market in 1997 after being linked to heart problems in users.
Jurors in the southwest Oregon town of Coquille concluded that American Home's drugs -- part of the discontinued fen-phen diet combination -- caused heart problems for Richard Wirt and Juanita Batson and that the company hid the drugs' risks.
Some drug companies shied away from the diet market, after part of the popular fen-phen diet combination was removed from the market in 1997 because it damaged heart valves.
The company, based in Madison, N.J., will have paid a total of $11.2 billion by the end of this year to help resolve litigation over the fen-phen diet combination, Mr. Martin said at the company's annual meeting with analysts in New York.
The diets were paired with four non-nutritive odours (coconut, banana, cinnamon, spearmint), with a unique odour and diet combination allocated to each group of four horses.
Only under one diet combination, resveratrol appears to increase mean lifespan of females but not at a statistically significant level after multiple comparison adjustment.
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