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In nature, carotenoids are found in a variety of environments ranging from hydrophobic and low dielectric lipid membranes and structures to sites at the interface with the polar aqueous medium of the cell.
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We find good agreement with dielectric models for lipid bilayers obtained by other approaches.
The paper mainly presents an extensive characterization of the fluorescence properties of the probes in organic solvents of different polarities and dielectric constants, and in lipid vesicles of varying compositions, focusing on the fluorescence response versus lipid phases, either solid, liquid ordered (raft) or liquid disordered phases.
We apply this method to determine the dielectric profile of a lipid bilayer surrounded by water.
Such a half-channel might work as an exit for the proton only if the channel was large enough to carry sufficient solvent, which has a much higher dielectric constant than the lipid bilayer or the protein.
Although single-molecule fluorescence detection has become routine in the laboratory, the light-scattering detection of single dielectric nanoparticles such as lipids, polymers, mesoporous silica, and virus-based drug delivery systems with sizes smaller than a few tens of nanometers remains a significant challenge.
The primary role of ion channels is to facilitate the movement of ions across the dielectric barrier imposed by the lipid bilayer.
Since solvent molecules generally penetrate deep into the lipid headgroup region, the dielectric constant in this area can reach much higher values than assumed in our work.
Both the macromolecule and the lipid membrane are treated as a low dielectric material, with dielectric constant εm = 2 within adsorbate and membrane.
The transfer free energy is therefore dependent on the dielectric property and the thickness of the lipid bilayer regions.
We note that attempting to describe dielectric properties of the interior of a lipid membrane by a uniform dielectric constant may not always be appropriate.
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