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When lights failed, it did not switch off.
To ensure that the re-introduction of NAT1 did not switch off expression of the shRNA, endogenous NAT1 mRNA was quantified by amplification of exon 4, an exon located in the 5'UTR of NAT1 mRNA but is absent in the plasmid used to rescue NAT1 activity.
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"Ryan [Moore] said to forget the race, he just didn't switch off.
We didn't switch off the whole time, someone would be DJ-ing while someone else was flaked out on the floor.
I forced myself on my friends so often that it's a wonder they didn't switch off the lights and hide in a cupboard whenever they saw me coming.
"They didn't switch off when they led 2-0 and that is not always the case.
Michael Segalov, a member of the NUS NEC, describes her as the same person onstage as off, he said, because she does not switch off.
The business however, does not switch off, and she is scathing about chief executives who are "experts in old ideas" and whose stores still follow the rhythm of a working day.
"Doors to automatic - do not switch off your electronic devices": British Airways passengers from Heathrow to Barcelona, Istanbul and Madrid on Thursday, will be the first to benefit from relaxed rules on gadgets inflight.
"Whoever does not switch off the adblocker or does not pay cannot see any content on Bild.de, as of now," the publisher said in a statement on Tuesday.
And no needless simplification, because anyone watching telly who does not switch off when they see a team of archaeologists is de facto announcing their readiness for a complicated conversation.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com