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Addition of a highly purified preparation of SR proteins fully restored splicing activity in reactions depleted of U1 snRNP but did not reconstitute splicing in reactions depleted of the other snRNPs.
To our surprise, 1-MT treatment did not prevent that Kyna levels increased in repeatedly stress mice and it also did not reconstitute the inducibility of TNFα in splenocytes.
The subject who was persistently weak did not reconstitute his motor network.
In contrast, V[1]-Ku80 did not reconstitute fluorescence with V[2]-Ku80.
By contrast, treatment with the PI3K substrate, phosphatidylinositol 4,5-bisphosphate (PI 4,5 P2), did not reconstitute superoxide production.
The co-transfection of all three mutant GMS sequences together did not reconstitute normal Irga6 behaviour in MIF-induced gs3T3-Irga6 cells.
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And yet the film's ending does not reconstitute the traditional family as such but rather suggests a more expansive and even progressive idea of what "family" might mean.
In PCA, a reporter protein such as a monomeric enzyme or a fluorescent protein (GFP or a variant thereof, IFP will be used as an example here) is rationally dissected into 2 fragments that do not reconstitute spontaneously [13], [14], [15].
Most importantly, the addition of Elba3 to the two ElbaC proteins (without the GST moiety) does not reconstitute full DNA binding activity.
Importantly, this result also confirms the specificity of the interaction, because the two fragments of the fluorescent proteins are present in the same cell but do not reconstitute the fluorophore if the orientation is not adequate.
This hypothesis is consistent with our findings that the fast cycling mutant Cdc42 F28L, which undergoes spontaneous activation without the assistance of GEF proteins, does not reconstitute Ca2+ mobilization in B6A4C1 cells (M.M.W., unpublished observations).
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com