Our initial hypothesis was that domain 2 of the protease was the main contributor to substrate specificity, dictating boundary cleavage.
Alignment of the amino acid sequences on the P′ side of the boundary reveals no clear amino acid conservation beyond the preference for smaller residues (glycine or alanine) in the P1′ position and lysine, glycine, glutamine or proline in the P2′ position, suggesting the P' side may have relatively little influence in dictating boundary cleavage.
The aim of the present study was to investigate whether domain 2 of the protease alone dictates boundary recognition and cleavage by exchanging individual domains from the MNV protease with the equivalent domain from the HNV SV protease to construct chimaeric NS6 proteases.
When using the Rayleigh Ritz method, the reference conditions also dictate the boundary conditions to be fulfilled by the shape functions used to describe the body's elasticity.
Along the thermal grooves, where the grain boundaries and the exterior surfaces couple, balance of mechanical forces, continuity of the surface chemical potential, and balance of mass flux dictate the boundary conditions.
These recent studies make it clear that relying on morphology alone to dictate species boundaries is not sufficient, and mating type analysis and molecular work is needed to correctly identify species boundaries and the relationships between species.
"They say, 'We're doing it to honor Native Americans and the history of the state.' But it just seems like misplaced atonement, especially when they want to dictate the boundaries of that atonement".
The analysis shows that the fuel mole fraction is a key parameter dictating the boundary between the modes of ignition.
Bluntly said, one could contend that your own digital traces dictate the boundaries of your personal reality, your bubble.
The omniscient daddy-state led by Ataturk and a string of successor generals dictated the boundaries of political, religious and cultural life and those who dared to contradict them suffered terribly.Nearly a century on this Kemalist straitjacket is in tatters, and from it has erupted what Jenny White, a professor of anthropology at Boston University, calls the "new Turks".
