Your English writing platform
Discover LudwigExact(1)
Muscle tissues of soleus and diaphragm were fixed in 4% paraformaldehyde/PBS overnight at 4°C, washed in PBS, dehydrated in increasing concentrations of ethanol and embedded in paraffin.
Similar(59)
A rat diaphragm is fixed on this membrane.
The E18.5 diaphragms were fixed in 4% PFA and dehydrated in 100% methanol.
These experiments were paralleled by a very successful collaboration between JND and Dr Andrew Engel at the Mayo Clinic whereby mouse diaphragms were fixed and sent to Minnesota, USA for freeze fracture and electron microscopy.
Briefly, for picrosirius red staining, OCT diaphragm sections were fixed in ice-cold methanol for 5 minutes at 4°C, washed with water and incubated in picrosirius red for 1 hour.
Diaphragm muscles with central tendons were fixed in 4% PFA for 20 min at RT before incubating for 90 min at RT with 8 μg/ml goat anti-mouse Lyve-1 antibody in cRPMI/0.05% Triton X-100 (Sigma-Aldrich, UK).
The meshes were 2 cm in diameter and were fixed to the diaphragm with four polypropylene (6-0) stitchestitchestitches
Quadriceps femoris muscles, heart, diaphragm, sciatic nerves and spinal roots from wild-type, dy3K/dy3K and dy3K/δE3 mice were fixed for 2 hours with 1.5% glutaraldehyde/1.5% paraformaldehyde, rinsed in Sörensen's phosphate buffer, post fixed in 1% OsO4 and then embedded in Epon.
The edges of the diaphragms have 'zero displacement' boundary conditions, so the diaphragms could be considered to be fixed supported.
The pressure range in the discharge tube is fixed by a diaphragm adjusted between the discharge and the post-discharge.
The fundus at the greater curvature of the stomach was fixed to the diaphragm with five interrupted nonabsorbable sutures in order to prevent the fundus from being folded and to keep the spleen fixed in the left upper abdomen (Fig. 4c).
Write better and faster with AI suggestions while staying true to your unique style.
Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com