The general puncture device was 3.5 mm in diameter, and we used a line 3.5 mm in diameter to simulate the puncture process (Line EI in Fig. 1).
To quantitatively describe the red shift of the plasmon resonance wavelength λLSPR with increasing gold nanoparticle diameter d, we used the allometric power law as follows: 2with k as amplitude, a as scaling exponent, and c as offset constant (for values, see Supporting Information SI 6).
To test for the presence of KNa current in small diameter DRG neurons, we used a method previously applied to rat DRG neurons (Bischoff et al., 1998): a K+ background current arising from defined pipette Na+ is measured using hyperpolarizing voltage-steps during the first 5 min following formation of the whole-cell recording configuration.
For all other species, and for large trees that exceeded the diameter range of species-specific diameter-to-ACD models, we used a general allometric model for tropical trees developed by Chave et al. [31] that uses diameter, height, and wood density to estimate ACD (Additional file 1: Table S4).
For all other species, and for large trees that exceeded the diameter range of species-specific diameter-to-ACD models, we used a general allometric model for tropical trees developed by Chave et al. [ 31] that uses diameter, height, and wood density to estimate ACD (Additional file 1: Table S4).
In order to obtain a more accurate value of the average diameter of the NPs, we used the modified Scherrer formula suggested by Ahmad et al. [27].
We used diameter of dry figs because it was the sole fig-size measure available for all species.
To test for associations between sexual system and either spore diameter or antheridium number, we used a ML approach in the discrete module of BayesTraits [ 38].
Because the diameter of the nanopore we used here is similar in size to the low molecular weight proteins, the adsorption of proteins inside the nanopore will cause a kinetic trap and minimize the diffusion caused by concentration gradients.
The internal diameter of the tube we used was somewhat larger than that of the cannula's cap, but the flexibility of the conjunctiva beneath the tube is able to block the internal space from the external atmosphere.
