Reduction of inner diameter transfers the 0 derivative point in the direction of the lower flow rate and increases the stability of the well's operating regime.
Free suspension spheroids of wild-type or transduced cells grown to ∼300 µm in diameter were transferred into 1% agrose gel and compressed as described before.
Briefly, spherical aggregates ranging in size from 450 650 µ in diameter were transferred to the inner chamber of the tissue surface tensiometer and positioned on the lower compression plate (LCP).
Colonies with 2 3 mm in diameter were transferred to solid SD-Ade-His-Leu-Trp medium and grown for 3 5 days at 30 °C.
Glass coverslips (15 mm diameter) were transferred into 6-well plates and wells were filled with cell culture medium.
In summary, stool specimen (5-6 mm in diameter) was transferred into diluent vial and mixed with sample diluent.
Six to 12 typical colonies (yellow and 1 to 3 mm diameter) were transferred to nutritive soft agar (T1N1, 0.75% agar) and incubated for 24 h at 37°C.
Two pieces of the harvested Kombucha BNC pellicle in 5 mm diameter were transferred into the fermentation medium of 50 mL.
Whole lungs were excised, cut into small pieces (max 0.5 cm diameter) and transferred in a sufficient volume of RNAlater (Ambion) and frozen in liquid nitrogen.
Single colonies typical of V. cholerae i.e. yellow (sucrose fermenting) and flat (2 to 3 mm in diameter) were transferred to 10% sheep blood agar.
