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Reduction of inner diameter transfers the 0 derivative point in the direction of the lower flow rate and increases the stability of the well's operating regime.
The centre of the plates was inoculated with 2 μl of a suspension of 500 spores/μl or with a small agar plug containing mycelium (1 mm diameter) transferred from the edge of a vigorously growing 3-days-old colony.
Cut out discs from the pastry about 12cm in diameter and transfer onto a floured tray.
On a floured surface, roll out the pastry to a rough circle, 2-3mm thick and about 35cm in diameter, and transfer to the baking tray.
We selected and grew het-R het-V or het-C2 het-E SI strains in permissive conditions (32°C or dihydrostreptomycin containing medium) for 24 h until the mycelia reached 1 cm in diameter, before transfer to non permissive conditions (26°C or dihydrostreptomycin free medium).
Free suspension spheroids of wild-type or transduced cells grown to ∼300 µm in diameter were transferred into 1% agrose gel and compressed as described before.
Briefly, spherical aggregates ranging in size from 450 650 µ in diameter were transferred to the inner chamber of the tissue surface tensiometer and positioned on the lower compression plate (LCP).
Colonies with 2 3 mm in diameter were transferred to solid SD-Ade-His-Leu-Trp medium and grown for 3 5 days at 30 °C.
Glass coverslips (15 mm diameter) were transferred into 6-well plates and wells were filled with cell culture medium.
In summary, stool specimen (5-6 mm in diameter) was transferred into diluent vial and mixed with sample diluent.
Six to 12 typical colonies (yellow and 1 to 3 mm diameter) were transferred to nutritive soft agar (T1N1, 0.75% agar) and incubated for 24 h at 37°C.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com