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Both proteins form compact spikes with a ∼10Å diameter tip that is stabilized by a centrally positioned iron ion bound by six histidine residues.
0.4 μg of sonicated VrPPase proteoliposomes (UP50H compact lab homogenizer (Hielscher), 1 mm diameter tip: 20%% amplitude, 10 pulses, pulse time=0.5 s) were combined with VrPPase SURFE2R buffer (100 mM HEPES-KOH (pH 8), 50 mM KCl, 5 mM MgCl2, 0.16 mM EGTA) to a total volume of 50 μl and applied directly to each sensor.
Twenty brine shrimp larvae were placed in vials containing extract using a plastic pipette with a 2 mm diameter tip.
A 5.0 prolene filament suture, treated with a glue coating to enlarge the diameter tip (375 µm) was used as an occluder.
Therefore, we constructed a 1 mm diameter tip for the present study.
The 5.5-mm diameter tip of the snout is placed in contact with the cervix, as described in our earlier publications.
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We also attempted to stimulate cultured cortical neurons using glass micropipettes with 5-10 μm diameter tips coated with CdSe QDs.
Ultrasonic probes with large diameter tips provide low ultrasonic energy density owing to a wide emitter surface [ 99].
The sites of cortical stimulation were placed about 10 mm apart, and DCS (0 10 mA, 50/60 Hz, 4 s duration) was carried out using a bipolar electrode with 1 mm diameter tips separated by a distance of 5 mm.
Furthermore, Fan et al. showed that dual-diameter NWs with a small-diameter tip and a large-diameter base exhibit an enhanced absorption over the wavelength range of 300 to 900 nm [16].
Bullets consisted of a lead core (68% of mass) and a copper jacket (32%); lead was exposed only at the 1.7-mm-diameter tip of the bullet.
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