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Each recording electrode was made by a polyurethane-insulated, copper nickel wire (50 µm diameter) inserted into a supporting polyimide tube (70 µm interior diameter, 86 µm outside diameter).
After 5 mm advancements, the osteotomies were fixed with screws 2 mm in diameter inserted with a torque control screwdriver at either 60° or 90° to the long axis of bone, in a matched pair experimental design.
To provide different degrees of belowground growth restriction, all lines were grown in both small (10 mm diameter) and large cylinders (40 mm diameter) inserted into standardised cells (65 mm diameter) within a flat completely filled with compost.
The soil mixture (4.5 kg) was packed in transparent plastic tubes (80 cm high × 7.5 cm diameter) inserted into opaque polyvinyl chloride (PVC) pipes.
The soil mixture (4.5 kg) was packed in transparent plastic cylinders (80 cm high × 7.5 cm diameter) inserted into beige polyvinyl chloride (PVC) pipes.
The two chambers were connected to a ventilator (Puritan-Bennett 840) via a cuffed endotracheal tube 8 mm in diameter inserted into the common tube.
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For each flow-through experiment, a new steel capillary (30 mm length; 6 mm outer diameter; 3 mm inner diameter) was inserted into the cylindrical rock samples (each 50 mm in length, 25 mm in diameter).
After reaming, a dilator with a diameter equal to the graft diameter was inserted to smooth and compact the tunnel.
One vial is sufficient for two T75 flasks and cells from two T75 flasks are enough for 18 24 Transwell 12 mm diameter inserts (1×10 cells/insert).
Migration assay was performed using the transwells (Corning 6.5 mm Diameter inserts with 8um pore size, polycarbonate membrane; product #3422, lot #19614003).
The cell migration assay was performed with a Transwell insert system (6.5 mm diameter inserts with 8.0 μm pores in a polycarbonate membrane situated in wells of 24 well polystrene, tissue culture-treated plates, Corning, Lot #21212046, USA).
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