Sentence examples for dialysis buffer from inspiring English sources

The dialysis buffer was then replaced with detergent-free buffer three times every 8 h.

The detergent concentration (initially 4%, w/v) was gradually lowered by doubling the dialysis buffer volume with detergent-free buffer every 8 h until the OG concentration in the dialysis buffer reached 0.25% (w/v).

After this step, the dialyzed sample was loaded on the MonoQ column (GE Healthcare) previously equilibrated with dialysis buffer.

Purified proteins were dialysed using DiaEasy Dialyzer (BioVision) in dialysis buffer (25 mM Tris, pH 7.4, 0.2 M NaCl, 0.5 mM EDTA and 2 mM dithiothreitol (DTT)) overnight at 4 °C.

Beads were washed twice with 1× dialysis buffer (2 mM EDTA, 50 mM Tris-Cl, pH 8.0) and four times with IP wash buffer (100 mM Tris-Cl, pH 9.0, 500 mM LiCl, 1% NP40, 1% Deoxycholate).

The His-tag was removed by overnight incubation with TEV protease at 4 °C in the dialysis buffer (25 mM HEPES, pH 7.9, 100 mM NaCl, and 2 mM DTT).

The dialysis buffer was changed four times to sufficiently allow VEGF165 refolding.

Eluted hDicer protein was dialyzed with TEV enzyme against dialysis buffer overnight.

All dilutions were done with the used dialysis buffer.

Substrate solutions were prepared in the final dialysis buffer.

The final dialysis buffer A contained 20% glycerol.