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Epoxy layer thickness was determined using Peacock dial thickness Gauge gauge (Ozaki MFG Co., Ltd., Japan).
The thickness of the skin piece (1.5 to 2 mm) was measured using a micrometer (Dial Thickness Gage, 2046 S, Mitutoyo Corporation, Kanagawa, Japan).
Changes in ear thickness for both sets of experiments were measured both before injection and 24 hours after injection using a dial thickness gauge (Swiss Precision Instruments, Carlstadt, NJ).
The thickness of irradiated skin was measured by 7 days of treatment by dial thickness gage (Mitutoyo).
Paw swelling was measured by using a dial thickness gage (Mitutoyo, Kanagawa, Japan) and was calculated as right paw thickness minus left paw thickness.
Ear thickness was measured 24 hrs after application of DNCB or mite extract with a dial thickness gauge (Kori Seiki MFG, Co., LTD., Japan).
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Because it is flat it does not distort the dial's features, so its thickness is not evident by looking at the watch straight on.
After removing the shell membrane, the shell thickness was measured using a dial gauge (Peacock, Tokyo, Japan) at three locations on the egg (air cell, equator and sharp end), and an average value of the three measurements was recorded.
Furthermore, hind paw ankle joint thickness was measured using a dial caliper (POCO 2T 0- to 10-mm test gauge; Kroeplin Längenmesstechnik, Schlüchtern, Germany).
Hind paw ankle joint thickness was measured using a dial caliper (POCO 2T 0- to 10-mm test gauge; Kroeplin Längenmesstechnik, Schlüchtern, Germany).
Change from baseline in paw ankle thickness was determined daily by dial calipers, and an average change in ankle thickness was determined for each mouse from the two hind paw measurements.
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