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"Last step is bringing this technology from research and drug discovery to diagnostic questions and personalized medicine.
Multiplex molecular assays are powerful tools that can provide rapid solutions to challenging diagnostic questions.
Screening and diagnostic questions based on ICHD-II were followed by burden enquiry in multiple domains.
Although diagnostic questions were included in the methods, the responses to these are not analysed here.
Demographic enquiry was followed by diagnostic questions based on ICHD-II criteria.
The diagnostic questions had been validated earlier in a Pakistani population [15].
Mean agreement for all other diagnostic questions was 78.1% (range 70.6-100%).
Diagnoses were not made by the interviewers but later, by algorithm applied to the responses to the diagnostic questions.
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Demographic, screening (for headache) and headache-diagnostic questions (based on ICHD-II [17]) were supplemented by several question sets addressing impact.
Screening questions for headache (with an enquiry timeframe of the preceding year) were followed, in those responding positively, by headache-diagnostic questions based on ICHD-II [14].
The questionnaire included headache-diagnostic questions based on ICHD-II, the Headache-Attributed Lost Time (HALT) questionnaire, and HADS for depression and anxiety.
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