This would require further analysis using nuclear gene sequences (e.g. transferrin) (there are no differential diagnostic microsatellite loci for the two species [ 17]).
However, we lack a fully diagnostic microsatellite loci and, along with an unresolved phylogenetic relationship of ER8* haplotype, restricted us from confirming this hybridization issue.
Hybrids based on: potential hybrids detected using a) N EWH YBRIDS software, b) diagnostic microsatellite alleles or c) cyto-nuclear disequilibrium.
We combined the data from mtDNA and three diagnostic microsatellite loci (ILSTS013, ILSTS050 and SPS115) to calculate the frequency of yak carrying cattle genes introgressed from mitochondrial and/or nuclear genome in each population.
Moreover, even in the two morphologically similar Acanthoscelides obtectus and A. obvelatus, no evidence of interspecific hybridization has been detected in natura, despite the genetic analysis (by means of diagnostic microsatellite loci) of hundreds of individuals from the two species, among which dozens originated from sympatric populations [ 15].
Artificial propagation and alteration of the physical landscape have resulted in human-induced changes to gene flow such that characteristically diagnostic neutral microsatellite markers may not discriminate seasonally migratory runs of Chinook salmon (Banks et al. 2000; Hedgecock et al. 2001; O'Malley et al. 2007).
Clades marginally overlap in some geographic areas (e.g., Napo River basin) but are reproductively isolated, evidenced by diagnostic differences in microsatellite PCR amplification profiles or DNA repeat number and coalescent analyses (in MDIV) best modelled without migration.
Three of these children harboured an identical NDUFS2 mutation (c.875T>C, p.M292T), which was also identified in conjunction with a novel NDUFS2 splice site mutation (c.866+4A>G) in a fourth Caucasian child who presented to a different diagnostic centre, with microsatellite and single nucleotide polymorphism analyses indicating that this was due to an ancient common founder event.
The final pre-assignment was made, however, using 9 diagnostic SNPs and 20 microsatellites [ 41] together with a large number of comparative samples, within an extensive pan-European hybridization survey [ 24].
With this aim, we characterized each wPip individual infection using two to five Wolbachia markers and identified Cx. pipiens members using diagnostic nuclear markers, including microsatellites for a subsample of populations.
