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A monitoring with spirodiclofen at a diagnostic concentration of 4 mg litre−1 revealed full susceptibility of multi-resistant T. urticae strains from Colombian roses.
After 1-hr exposure to diagnostic concentration of deltamethrin (0.05%), mosquitoes were transferred into insecticide-free tubes and maintained on 6% sucrose solution for 24 hrs.
The same treatment killed 100% of FRA and F1 larvae, indicating that resistance was completely recessive at the diagnostic concentration.
Both strains had dominant resistance to a diagnostic concentration of Cry1Ac in diet and to Bt cotton leaves containing Cry1Ac.
The F1 progeny were tested at the diagnostic concentration of Cry1Ac, and survivors were reared to adults.
We adjusted for control mortality (range = 0 8.3%) to estimate LC50 values, but not in the bioassays with only the diagnostic concentration, which had lower control mortality (<5%).
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The following diagnostic concentrations of insecticides were tested: 4% DDT, 0.75% permethrin, and 0.05% deltamethrin.
The diagnostic concentrations to ascertain genotype frequencies were determined from the concentration-mortality curve of the F2 generation.
More importantly, it will allow multiclone follow-up and the detection of emerging subclones at diagnostic concentrations far below that of the main clone identified at diagnosis, as well as full repertoire analysis [ 33– 33].
In conclusion, and in agreement with other cohorts, we observed associations between pre-diagnostic concentrations of total and free testosterone in premenopausal women and risk of breast cancer.
Pre-diagnostic concentrations of IGF-I (nmol l−1) were analysed with enzyme-linked immunosorbent assays at IARC (phase 1 (Peeters et al, 2007): DSL, Webster, TX, USA) and at the Division of Cancer Epidemiology at the German Cancer Research Center (phase 2: Immunodiagnostics Systems, Frankfurt, Germany).
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