Intermediate zone was practically devoid of nuclei marked by the expression of H4R3me2s.
These cloacal nerves terminate in two large, serotonin-like and allatostatin-like immunoreactive, platelet-shaped structures devoid of nuclei.
Unlike in the distal tip where they first multiply in the surrounding somatic gonad, the few Wolbachia leading the invasion of the germ cells toward the proximal end of the ovary are detected in the center of the gonad, often in actin-rich areas devoid of nuclei, the rachis (supplementary material Movie 11).
Although devoid of a nucleus, they retain low levels of mRNA which undergo translation.
Nucleated cells expressing CD41 as well as small cytoplasmic fragments devoid of the nucleus (presumably blood proplatelets/platelets) were also detected within blood islands (Figs. 3a, b, d, 4a, b, e, f, g, j, k, l, o).
However, they must also possess some commonalities as each results in the synthesis of a cell containing a complex cytoskeletal network, filled with hemoglobin, and devoid of a nucleus and internal organelles.
Platelets are devoid of a nucleus and genomic DNA, but they were shown to contain subcellular organelles, such as rough endoplasmic reticulum and ribosomes [ 60], as well as a small amount of poly(A) + RNA from their megakaryocyte progenitor cells [ 61], sufficient to support de novo protein synthesis.
Sections stained with H&E were used to visualize generalized chondrocyte cell death (determined by the amount of lacunae lacking discernable nuclei), localized cell death/focal cell loss (the presence of distinct regions devoid of discernable nuclei) and chondrocyte cluster formation (lacunae containing more than one nuclei).
Decellularization protocol yielded aortas devoid of cell nuclei, with preserved structure, including a preserved basement membrane.
A TUNEL assay was performed to detect DNA fragmentation but all sections of MDA/EGFP and MDA/RLN2 tumours were devoid of apoptotic nuclei (data not shown).
