In this study, we show for the first time that it is feasible to purify a population of hESC-derived hepatic progenitors that are devoid of viral integration and that can differentiate further into more mature hepatocyte-like cells.
By combining 1) cell sorting using a hepatic-specific promoter, 2) high-titer preparations of purified ILVs and IDLVs, and 3) a specific integrase inhibitor, we created a robust and highly efficient method for purifying hESC-derived hepatic progenitors devoid of DNA integration.
An additional 165 genes were almost entirely devoid of transposon integrations in all three Salmonella strains examined, including many genes associated with protein and DNA synthesis.
Moreover, they were devoid of vector DNA integration.
Both the dax1 and foxl2b promoters appear devoid of any transposon integrations.
For example, DA neurons projecting to dorsolateral DS, which is involved in sensorimotor integration, are largely devoid of ERβ and AR, whereas DA neurons projecting to associative regions of the DS express ERβ and those projecting to the NAc primarily express AR [ 219].
Some chromosomal regions were essentially devoid of CfERVs whereas other regions had large numbers of integrations in agreement with distinct selective pressures at different loci.
This would imply that the integration of vector backbones, even devoid of transposase, may be a frequent but generally overlooked event in piggyBac transformation.
Such particles are devoid of Vpr and retain their transgene-transferring capability and integration proficiency, with the latter property depending on the vector-contained IN-protein composition [ 8, 10].
Furthermore, we know of numerous integrations of retroelements [ 10] that make the chromosome region that is devoid of replication origins even larger, assuming origins of replication do not integrate in the nuwt along with retroelements.
We defended ever-deeper integration and ever-wider federalism; a uniform, homogeneous Europe, devoid of identities.
