The fact that Oct4+/− cultures not only lack cells expressing the highest Oct4 concentrations present in Oct4+/+ cultures but are also essentially devoid of the differentiated Oct4-negative cells that are readily detectable in WT cultures is of no small interest here.
Carcinomas of the alveolar subtype (G2) and poorly differentiated carcinomas (G3) were pure NE carcinomas, devoid of apocrine differentiation.
To verify the dependence on LIF, we used the population of C5L, that was devoid of clumps and differentiated trophoblast, characteristic for B10L.
The chromatin of pluripotent stem cells was noticeably devoid of heterochromatin, though prevalent in differentiated cells, suggesting that ES cells have an open or "loose" chromatin structure.
This is nicely illustrated at 30 hpf, where strong GFP expression is detected at the base of the forming islet where Nkx6.1+ pancreatic progenitors are located, while weak GFP labeling is found dorsally within the islet, where differentiated endocrine cells, devoid of Nkx6.1, are clustered (Fig. 1c).
Sorting and investigation of all observed cell populations revealed that only the population indicated in the FACS plot in Figure 1A (differentiated cells) was devoid of larger, Mira-positive cells, and contained small ELAV- and Pros-positive cells (data not shown) but never any NBs.
In contrast, TRAF4 is never localized at the plasma membrane of poorly-differentiated invasive carcinomas devoid of correct TJs, but is observed in the cytoplasm and/or nucleus of the cancer cells.
Our approach, based on purified IDLV, facilitates the generation of a population of purified hESC-derived hepatic progenitors, which are devoid of integrated viral DNA, and can be subsequently differentiated to mature hepatocyte-like cells.
Normally, chondroprogenitor cells synthesize predominantly exon 2-containing mRNA isoforms (type IIA and IID) while Col2a1 mRNA devoid of exon 2 (type IIB) is the major isoform produced by differentiated chondrocytes.
