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Well, there's more in common with the physiology of jellyfish and the "bloom" of school leaders than you might think: both of them are utterly devoid of a backbone.
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We demonstrate for the first time that DNA minicircles (small DNA vectors encoding essential gene expression components but devoid of a bacterial backbone, thereby reducing construct size versus conventional plasmids) deployed with magnetofection achieve the highest, safe non-viral DNA transfection levels (up to 54%) reported so far for primary NSCs.
Moreover, MIDGE-vectors are devoid of plasmid backbone DNA and therefore have a smaller molecule size, so their effective transgene dose per microgram DNA is much higher than that of conventional plasmids [ 30].
We set out to improve SB gene transfer into hard-to-transfect human CD34+ cells by vectorizing the SB system components in the form of minicircles that are devoid of plasmid backbone sequences and are, therefore, significantly reduced in size.
Purified DNA fragments devoid of vector backbone were injected into mouse pronuclei to generate transgenic mouse lines in the facility of Zentrum fuer Molekulare Biologie at the University of Heidelberg.
Thus, no classical phenotype found in mutants devoid of OPGs backbone could be associated with mutants only devoid of substituent.
The main phenotypes of mutant strains devoid of OPGs backbone (i.e., opgG or opgH) are loss of motility and increased exopolysaccharides synthesis (see Section 1).
Minicircle DNA vectors devoid of bacterial plasmid backbone sequences overcome transgene silencing providing persistent transgene expression.
He has more of a backbone.
You've got to have a bit of a backbone".
"The confession is usually devoid of a lot of facts.
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