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The experimental setup was devised to assay one male and one female from each cross and temperature per day (five blocks) for both Tp and Tko, amounting to 1,080 flies in total.
With the advent of "systems modeling", a diverse set of methods have been devised to assay the interactions, both physical and functional, among different active entities in the cell, including protein-protein [ 14– 16], protein-DNA [ 17, 18], and genetic [ 19– 21] interactions.
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First, a microplate-based binding assay was devised to characterize the binding of effectors to immobilized synthetic PIPn headgroup-biotin conjugates corresponding to all seven isomers.
A quantitative real-time PCR (qPCR) assay was devised to determine the TYMS gene expression level.
A TaqMan assay was devised to detect wild-type splicing between the exons on either side of the mutagenic cassette.
This assay was devised to determine whether incubating the toxin, free in solution, at low pH, would result in premature conformational changes that result in unfolding and inactivation (i.e., entry into an "off pathway").
Primers and probes of this study were devised to be suitable for multiplexed assays but these evaluations await to be performed.
As described below, secondary assays were devised to distinguish these mechanistic possibilities.
In the present study, electrophysiological assays were devised to monitor cytoplasmic free Ca2+ in single frog olfactory cilia.
The methods described were devised to enable the development of an assay concept that could be scaled up to achieve high-throughput reliable multiplexed analysis of samples.
Various PCR based assays have been devised to measure HPV copy number and physical state in cell lines and clinical samples [ 5- 13], with a view to deriving clinically or biologically useful information.
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CEO of Professional Science Editing for Scientists @ prosciediting.com