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The plotted deviations of the reference algorithms are smoother due to the more frequent estimation rates in contrast to the adaptive estimation times returned by the bare AAC algorithm.
The DHPLC protocol we set up provided reliable results (see standard deviations of the reference curve in Fig. 1) and was reasonably sensitive.
The computations started with a standardization of the MPI-S scales using the mean values and standard deviations of the reference group to form Z-scores and then T-scores.
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For all these proteins, with molecular mass up to 25 kDa, COMPASS distinguished the correct fold, most often within 1.5 Å root-mean-square deviation of the reference structure.
The reliability of the models was assessed using the coefficient of determination in validation (R2V) and the ratio of standard deviation of the reference data in the validation set to the standard error of prediction (RPDV).
Z-scores were calculated for each metabolite based on the mean and standard deviation of the reference variety Nipponbare.
Here, μt is the sample mean and σt the standard deviation of the reference values.
Individual tumor sample gene expression was standardized using the mean and standard deviation of the reference.
For standardizing the factor mean differences, we used the pooled standard deviation of the reference group (FACE) and the comparison group (CATI or SELF).
All variables were centered at the mean of the reference group and divided by the standard deviation of the reference group.
The mathematical model used is based on the PCR efficiencies and the mean CP deviation between the sample and control group of target genes and is normalised to the mean CP deviation of the reference genes [ 83].
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com