Sentence examples for deviation of the spots from inspiring English sources
In contrast, the Imin metric moves these spots to the low end of the x-axis, and the curve fit tracks the standard deviation of the spots much better.
All pixels within approximately a half radius of each spot were used to calculate the background mean and standard deviation of the spot.
While under the green laser illumination, there is about 30-nm deviation of the position of the spot along the x-axis.
These clusters of protein spots are shown in Figure 3 and 4, in which log-transformed expression values of the biological replicates of different samples are shown in color [See additional file 2 for the averaged spot intensities and the standard deviation of these spots in each tissue and cell line].
A surrogate raw value is assigned when the foreground value does not exceed the background value by two standard deviations of the spot's background pixel values.
Differential expression was calculated through the mean and standard deviations of the spot distribution of Log2 ratio) values, and also defining a global fold change difference and confidence, equivalent to a z-test [ 68].
These results are supported by the plots for the standard deviation of the replicate spots.
With pre-hybridization, signal intensities were almost 50% higher and the mean standard deviation of the triplicate spots 15% lower compared to the protocol without pre-hybridization resulting in good CGH profiles of FFPE material (data not shown).
First, the change in level of expression of equivalent spots had to reach significance using the standard deviation of the normalised spot volumes and also a Student's t-test comparing Gefitinib-treated and DMSO-treated gels.
Spot net intensities were calculated from the median spot and background intensities with all results less than one standard deviation of the respective spot background pixel intensities ignored (set to zero).
Normalisation was then undertaken and then statistical analysis of the results was performed to display the standard deviation of the normalised spot volumes, and a Student's t-test was undertaken to compare Gefitinib-treated and the DMSO control spots.
