To determine the detection limit, the standard deviation of the baseline noise signal was multiplied by 3. The detection limit was 20 μM.
Equations 1 and 2: Mean and standard deviation terms in a standardized score (z-score, top) are replaced with the mean of the baseline, μ(b), and standard deviation of the baseline, σ(b).
The threshold cycle (Ct) is defined as the fractional cycle number at which the fluorescence reaches 10-fold standard deviation of the baseline (from cycle 3 to 10).
Standardization was accomplished by subtracting the mean baseline PLV from the PLV for each data point and dividing by the standard deviation of the baseline PLV.
As for quantitative analysis of evoked activation in image frames, the mean and standard deviation of the baseline activity of each pixel across the 50 frames preceding photostimulation was first calculated, and then activated pixels were measured.
Return to baseline was defined as the start of a series of 3 consecutive beats in which the blood pressure was within one standard deviation of the baseline blood pressure.
SDBaseline is the standard deviation of the baseline assessment.
* Effect size: the mean change score divided by the standard deviation of the baseline score.
The former is equal to the mean change in score divided by the standard deviation of the baseline scores [ 10].
Effect size was defined as mean score change divided by the standard deviation of the baseline score [ 22].
This set of mean firing rates is used to estimate the standard deviation of the baseline activity.
