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Modern ion chromatography standard methods [37] allow a deviation of retention time up to ±10%% within a batch.
Positive identification of OC was based on LC retention time compared with that of OC-D3 surrogate standard, and < 0.05% deviation of retention time was tolerated.
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The RSD (relative standard deviation) values of retention times and peak areas of 6 successive runs were 0.5 0.7% and 1.8 2.8% for the separation of biogenic amines, respectively, and 0.1 0.2% and 2.1 3.0% for the separation of cations, respectively.
After alignment, the relative standard deviation (RSD) of retention time and peak area of each aligned peak were respectively calculated for sample groups 10 μg/mL, 25 μg/mL and 40 μg/mL.
According to the inferred formula of the xenon outflow curve for the six-pulse injection, the inferred retention time is 243 min, the relative deviation of the retention time is 4.7%, and the inferred peak width is 225 min, with a relative deviation of 1.3%.
The evaluation of about 80 measurements of reference mixtures with seven different MCC-IMS showed an improvement of the aligned data over the entire retention time range (5 300 s) with regard to the standard deviation of the retention time (Table 4).
The suggested variation is plus or minus three times the standard deviation of the retention time for each compound for a series of injections [49].
The deviation of the retention time is significantly reduced over all eight substances from 22.2 to 10.0%.
The correlation of a bioaffinity peak with an EIC peak is achieved with high certainty because a margin of twice the maximum relative standard deviation of the retention time, in this case ca 30 s, is applied.
Because the distribution of TSGD and LSP paralogs was homogenous, we use the ratio for each chromosome as a pseudoreplicate to calculate the average and standard deviation of LSP retention in Ostariophysi and Acanthopterygii lineages.
The average and standard deviation of the retention time were calculated for each standard and the LIC1 nucleotide (n = 5 for LIC nucleotide, n = 9 for GDP, n = 5 for GTP, n = 3 for ADP).
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