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Relative standard deviation of replicate samples (n = 6) was 2.19%.
The accuracy (% difference from nominal) and precision (% relative standard deviation) of replicate injections were within 17.0%.
Error bars represent the standard deviation of replicate measurements/batch (n = 3). Figure S3.
The detection limit (LOD) for the proposed methods was calculated using the following equation according to IUPAC definition (23): LOD = 3s/k; where s is the standard deviation of replicate determination values under the same conditions as the sample analysis in the absence of the analyte, and k is the sensitivity, namely the slope of the calibration graph.
LOD and LOQ were calculated as 3.3 σ/s and 10 σ/s, respectively, as per ICH definitions, where, σ is the mean standard deviation of replicate determination values under the same conditions as the sample analysis in the absence of the analyte (blank determination), and s is the sensitivity, namely the slope of the calibration graphs.
The precision of the method is then assessed via the relative standard deviation of replicate spike recovery values in the chosen field matrix.
Similar(53)
Error bars represent the standard deviation of replicates (n = 3).
The assays were tested in triplicate, and values are presented as mean ± standard deviation of replicated measurements.
Results are expressed as mean values ± standard deviation of replicates.
Error bars represent standard deviation of replicates.
The following default filtering parameters were retained: signal to noise ratio less than 3, standard deviation of replicates greater than 0.1 and exclusion of clones with missing values in over 50% of the tumors.
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