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Variation between PCR replicates and deviation of normalization factors were checked and outliers with values outside 2 SDs from the mean were removed.
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The criterion for convergence of MedianCD normalization was to require that the relative changes in the standard deviation of the normalization factors were less than 0.1%, or less than 10% for 10 steps in a row.
Signals were log transformed and standardized to a mean of 8 and a standard deviation of 2. RMA normalization was also applied to the lung data (Affymetrix M430v2).
It also discusses the analysis of parameters that includes explained statistical analysis, suppression of the correlation between parameters, systematic deviations and normalization of experimental data, physico-chemical testing, and significance of kinetic parameters and model discrimination.
Median absolute deviation scale normalization was used to normalize between arrays [ 24].
All computations were related to analyzing microarray data, and included the calculation of log-ratios and standard deviations, normalization of data, and the application of a t test, which the students performed using a simple handheld calculator (see Figure 1B for examples).
We implemented the Taylor et al. [13] version of the population vector method with one slight modification: the baseline firing rate (mean) and normalization constant (standard deviation) of neurons were fit once from training data, instead of updated during filtering using a sliding window of spiking history.
Mean and standard deviation of all experiments were calculated after normalization to PR-P0.
The normalization by the standard deviation of the image reduce this effect.
Inside this window, the standard deviation of M values was calculated and lowess normalization curve was estimated by using only data points whose M values fell into a range within three times the standard deviation in each A segment.
Sets the target of normalization.
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