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The next developments of our method will discard the contributions of the neighboring links L10−11, L12−13 and so on, and our considerations will be concentrated only on the target link L11−12.
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Following the development of our method, we had the opportunity to test it on a positive blood culture originating from a kidney transplant recipient who developed lymphoma in 2009 with a worsening condition.
We initiated the development of our method using spatial normalization within statistical parametric mapping (SPM).
During the development of our method, we tested the hypothesis that synonymous and nonsynonymous mutation counts alone have sufficient power to resolve functional versus nonfunctional proteins.
The next step in the development of our method is identifying specific efforts during research formulation, production and extension which aim to enhance the contribution of research to action.
As a next step in the future development of our method we aim to extend the protocol by allowing single-channel data, or dual-channel data for which intensity values are not available.
This lack of ability to discriminate populations underlies the necessity of formulating a null hypothesis via nucleotide mutation frequencies, and confirms the supposition of Deminoff et al.. Complete details of the experimental system and data analyzed in conjunction with the development of our method are presented in Kleinstiver et al. [ 20].
It is therefore crucial that we in headache research continue in our efforts to find these answers, through continued development of our methods and providing open-minded thinking to embrace new techniques and ideas into our field.
We use excerpts from our research team's reflexive statements to illustrate the development of our methods.
The remaining data of 1,676 mutations in 70 different proteins (S1676) were used for the development of our methods.
1) First, several of the predicted miRNA targets identified with our approach have also been predicted by other target prediction algorithms, which provide some support for the development of our methods.
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