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With the developments of molecular techniques, such changes can also be detected in the plasma or serum of cancer patients and, thus, may open up the potential of utilising circulating nucleic acids as a new generation of tumour markers.
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For example, the development of investigation methods has involved the development of molecular techniques in soil microbiology.
The recent development of molecular techniques such as massive parallel pyrosequencing will greatly contribute to this task.
Second was the development of molecular techniques that allow detection of fastidious bacteria including anaerobic bacteria without the need for culture [9], [31], [32].
The development of molecular techniques for detection of bacterial DNA in tissues has made it clear that bacterial culture methods underestimate the prevalence of bacteria in placenta and amniotic fluid [29].
The high incidence of local and regional recurrence despite complete resection as assessed by conventional pathologic assessment demonstrates the need for development of molecular techniques to evaluate margins in this disease.
The development of molecular techniques now allows a more accurate analysis of large collections.
The development of molecular techniques facilitated the detection of novel viruses in patients with respiratory infections.
The development of molecular techniques in diagnosing has brought its epidemiological impact into sight [ 7].
Development of molecular techniques and methods enables better understanding of processes underlying mammalian reproduction on cellular level.
Many of the limitations of conventional cytogenetic analysis have been overcome with the development of molecular techniques including FISH and comparative genomic hybridisation (CGH).
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