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Whether conidiation truly starts before the temperature transition or is simply an acute developmental effect of temperature on already existing, developmentally competent mycelia that are close to the growth front when the temperature transition occurs, we cannot say.
Developmental competence of the oocyte correlates with follicular size, larger oocytes being more developmentally competent [ 8, 9].
Therefore, refinements for embryo evaluation are needed that select for the most developmentally competent embryo.
Embryos resulting in a viable fetus at 11 weeks gestation were considered developmentally competent.
To determine whether the preantral follicles in adult ovaries can generate developmentally competent oocytes after in vitro culture.
To create developmentally competent embryos from failed-to-fertilize oocytes for use in infertility research and human embryonic stem cell derivation.
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However, a growing body of evidence, including the isolation of germline stem cells (GSC) from adult mouse ovaries that produce developmentally-competent oocytes, has challenged this belief.
However, recent studies [ 9], including isolation of germline stem cells from mouse ovaries that can generate oocytes in vitro [ 10] and developmentally-competent eggs in vivo [ 11], have essentially invalidated this longstanding dogma (reviewed in [ 12, 13]).
This model raises numerous questions, including whether growing follicles containing developmentally incompetent NSN oocytes may be rescued, that is, guided to acquire the SN chromatin organisation and whether this is paralleled by the acquisition of a developmental competent state.
This was done because oocytes cultured in groups have been observed to be developmentally more competent than oocytes kept in individual culture [ 27, 28].
In addition to the technical advantages, blastocyst stage biopsy has the advantage of selecting developmentally more competent embryos for diagnosis that could improve pregnancy rates while at the same time it decreases the cost of the PGD study [ 14].
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