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A multi-laboratory developmental validation study was performed on the PowerPlex® Fusion 6C System.
To ensure the efficacy of these markers, developmental validation studies were performed to determine the conditions and limitations of this new tool for forensic analysis.
Tests were designed to address the developmental validation guidelines issued by the Scientific Working Group on DNA Analysis Methods (SWGDAM), and those of the DNA Advisory Board (DAB).
The 10-plex was satisfactorily subjected to developmental validation tests following the SWGDAM guidelines and shows potential for its application to genealogical and evolutionary studies.
This developmental validation work follows the SWGDAM guidelines and demonstrates that the assay is robust and suitable for use on forensic casework and database samples.
This paper describes the developmental validation study performed on the PowerPlex® ESI 16 (European Standard Investigator 16) and the PowerPlex® ESI 17 Systems.
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The derivation of health states was split into developmental and validation phases.
A split technique was used to divide the overall sample into two groups: developmental and validation.
Frequency analysis and descriptive statistics were used to analyze the demographic characteristics of the developmental and validation samples, after excluding missing values.
Apical meristems from additional bulbs were sampled at each time point for developmental stage validation under a stereo microscope (Stemi 200 °C, Zeiss, Germany).
The Goodness-of-fit test was used to evaluate model calibration; discrimination was evaluated using area under the receiver operating characteristic curve (ROC), in the developmental and validation samples.
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