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Multiple adult features have led previous investigators to suggest that the behavioral castes of primitively eusocial wasps are preceded by developmental divergence during larval growth [10].
However, the data are based on adult sample material rather than larvae, thus they do not directly test the developmental divergence hypothesis.
We also used a non-targeted proteomics screen to test for peptide/protein abundance differences that could reflect larval developmental divergence.
Because the larval developmental divergence that we hypothesize could incorporate genes related to diapause and to caste differences in highly eusocial insects, we had searched literature to find candidate genes in those two categories.
Because diapause physiology may underlie developmental divergence in Polistes [21], literature was searched for genes differentially expressed in non-diapause vs. diapause phenotypes in other insect taxa (Table 1).
Our third major finding is the differential expression in a primitively eusocial paper wasp of some of the same genes and peptides/proteins that are implicated in worker/queen developmental divergence in highly eusocial bees.
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However, these extreme developmental divergences do not seem to be associated with a similar extent of molecular innovation in upstream patterning systems, as common transcriptional expression profiles are observed during the early stages of gut assembly among different ecdysozoan lineages.
Although not conclusive, our results support hypotheses of 1) larval developmental pathway divergence that can lead to caste bias in adults and 2) nutritional differences as the foundation of the pathway divergence.
Body size differences in fish have been linked to developmental stage divergence and transcriptomic differences have been detected between size and age matched wild rainbow trout.
It involves differences in reproductive timing and can be the result of behavioral or developmental schedule divergences [ 3, 4].
We also investigated pathways and gene regulatory networks that might involve in the developmental control of muscle divergence.
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