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Here we designed high-throughput developmental assays in the social amoeba Dictyostelium discoideum to measure Pseudomonas aeruginosa virulence and to screen for novel antivirulence molecules without side effects to the host and its beneficial bacteria Klebsiella aerogenes.
Therefore, compounds were selected that produced androgenic or anti-androgenic effects in reproductive and developmental assays.
Therefore the outcome proposed, deriving from 8 treatments and 2 developmental assays performed on 10 different types of mammalian cells, can be likely regarded as general for mammals.
Going forward, researchers who rely on suspension cAMP treatment to prime cells for chemotaxis or other developmental assays may benefit from knowing exactly how genes and processes behave relative to solid-support development.
They can also be cultured at high or low density, and in specific combinations, to study the intrinsic physiological properties of these cells or to investigate how cells of one type interact with cells of other types in a range of developmental assays.
For developmental assays the proportion of worms that had reached the L4 stage was determined at 12 hr intervals.
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The aim of this study is to examine the toxicity in the developmental stages of H. elegans using the method of developmental assay according to Gopalakrishnan (2002) and Gopalakrishnan, Thilagam, and Vivek Raja (2008).
Termed the "triple response, " this developmental assay has been extremely useful in genetically dissecting the role of various genes involved in ethylene synthesis or signaling in Arabidopsis [ 23].
For developmental assay each of the 20 BPH, GLH and WBPH first instar nymphs were introduced separately on each plant confined in an insect proof cage and mortality of insects was monitored at 3 day intervals for 24 days [ 23].
Most developmental neurotoxicity assays give reliable and specific results only when compounds are used at concentrations that do not trigger general cytotoxicity/cell death.
Following the procedure suggested for developmental neurotoxicity assay development (Crofton et al. 2011), we used the MINC assay to test > 20 compounds, including negative controls, end point specific controls, general developmental neurotoxicity compounds, and chemicals known to specifically impair NC cell migration in vivo.
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