Our developmental approach, which included developmental arrays and in situ hybridization using the pygmy squid embryos, indicated the contribution of camera eye-specific genes to the eye developmental process.
A mouse developmental tissue array, including triplicate 600 μm cores of brain cortical tissue from wild-type C57BL/6J and Mecp2tm1Bird/y for a number of age-matched timepoints including P7, P28, P35, P49, P56 and P70, was fixed and embedded in paraffin as previously described (72).
CytoSure Constitutional v3 arrays deliver the most advanced, high-resolution developmental disorder arrays currently available.
Margriet von Kogelenberg (Wellcome Trust Sanger Institute) described the UK-based Deciproject Developmental Disorders (DDD) project, which is currently recruiting 12,000 patients with developmental delay for array comparative genomic hybridization analysis and exome sequencing.
They treated Arabidopsis seedlings with chemicals that inhibit the function of HSP90 genes and produced a stunning array of developmental changes.
Uncontrolled FGF signaling is responsible for diverse array of developmental disorders, most notably skeletal syndromes due to FGFR gain-of-function mutations.
The Drosophila melanogaster gap gene system, in which model parameters are optimized against in situ immunofluorescence intensities, demonstrates the importance of error quantification, which is applicable to a wide array of developmental modeling studies.
In addition to the expected impacts on lipid metabolism, manipulation of these genes in mice is found to affect a wide array of developmental and physiologic processes ranging from interferon signaling in macrophages to synaptic transmission in the brain.
At intermediate levels of disturbance, the forest displays the broadest array of developmental pathways, highest entropy as measured by Shannon's index of diversity, and critical slowing near steady states.
