These results provide the foundation for future studies of mimicry, which should integrate phylogenetic and developmental analyses of wing pattern formation.
DRC directed the research project, advised on phylogenetic and developmental analyses, contributed substantially to the interpretation of the data and writing the manuscript.
Measurements with appropriate statistical tools can become a foundation for further studies, such as association mapping, comparative developmental analyses, genetic screens of functional tests and fitness assays.
While multiple previous studies have examined a few electrophysiological properties of this neuron type across development, the current study is distinct in providing: 1) comprehensive characterization of numerous sub- and suprathreshold properties, and 2) multivariate analyses of the developmental changes.
For this reason, Day-7 post-sporozoite populations were critical to our analyses of the developmental transcriptome because they represent the earliest observation of the growth-shifted population that ultimately commits the parasite to bradyzoite differentiation.
In their detailed analyses of the developmental defects of Bat3-deficient mice, Desmots et al. reported that these mutants showed impaired branching of the terminal bronchiolar alveoli in the lung as well as abnormal renal branching in the kidney, two processes for which BMP signaling is known to be crucial.
The use of time lapse photomicroscopy linked with qPCR data on chlamydial chromosomal DNA and plasmid replication provides a reproducible framework within which to begin molecular analyses of the chlamydial developmental cycle.
For developmental analyses, mouse pups were taken at different postnatal days, defining the day of birth as P0.
This is likely a result of the technical challenges historically encountered by those performing developmental analyses in mosquitoes.
