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For developmental analyses, mouse pups were taken at different postnatal days, defining the day of birth as P0.
This is likely a result of the technical challenges historically encountered by those performing developmental analyses in mosquitoes.
Excellent descriptive analyses of Ae. aegypti embryogenesis were completed in the 1970's [5], [6], and additional developmental analyses in this species were recently published [7], [8].
In addition to studies of plant gene function and developmental analyses, plant biotechnological use is largely dependent upon transgenic technologies.
Only children delivered at term (> 258 days) and conceived by 11 September 2001 were included in the developmental analyses.
A mouse sec8 knockout has been created, but dies early in embryonic development [ 16], precluding significant functional or developmental analyses.
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In the cross-developmental analyses here there were no significant patterns of differential expression in vermilion; instead it showed high, ubiquitous expression early in pupal development (before optix expression), progressively dropping lower over time to barely detectable levels during the ommochrome and melanin stages.
For developmental timing analyses, 30 larvae were placed per vial and pupariation timing was scored periodically.
Such cell systems would allow developmental toxicological analyses also on tissue-specific progenitor cell populations.
UM, MS and FL performed the dsx developmental expression analyses, and MS performed the Aearp49 developmental expression analysis.
The transcriptomic information available at the BOGAS T. urticae website [ 50] was used to the developmental expression analyses.
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