In order to identify constitutively expressed UCEs (i.e. with stable signal across development) we used a method previously developed by our lab [ 25].
Therefore, to develop a systems-level understanding of Hdac1 function during embryonic development, we used a global gene expression profiling platform to identify the Hdac1-regulated transcriptome in developing zebrafish embryos.
In order to test the hypothesis that only select sub-populations of cells within the developing non-retinal epithelia are of sufficient cellular plasticity to support eye development we used the UAS/GAL4 system to conduct the following screen.
To identify new interacting partners of TTG1 in seed development, we used the recombinant MBP-TTG1 protein to pull down its interacting proteins in total protein extracts from Arabidopsis Col-0 wild-type siliques.
To investigate the role of Dicer and microRNAs in neuronal polarity development, we used mice in which the RNase III domain of Dicer was conditionally floxed.
As we were interested in rapid development, we used daily Spanish and international news from different web pages.
To specifically address how subplate loss affects cortical development, we used in vitro mechanical subplate ablation in slice cultures (n ≥ 3) and found EPO treatment attenuates KCC2 loss.
To describe how many PhD theses and MSc dissertations were related to agile software development, we used the scriptLattes tool [42] to extract ongoing/concluded supervisions.
For model development, we used 19,557 GPS locations across 28 female sage-grouse; all locations were used for estimating resource selection of the non-behaviorally adjusted model.
To determine whether activins are involved in lung development, we used a reverse transcription polymerase chain reaction (RT-PCR -based appRT-PCR -basedtify membeRT-PCR -basedivin receptors from bapproachtol lung mRNAs.
To better understand the relationship between liver CD34+CD146+ and CD34+CD146- subsets and any effects of disease on CD34 development, we used gene expression profiling and computational modeling to compare each subset during ALD and HCV.
