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Color developed by the addition of o-phenylenediamine substrate, and color development was stopped by the addition of 4 M H2SO4.
After 5 min the color development was stopped using 2.5 M sulphuric acid and the color developed recorded with a Bio-Rad 680 microplate reader at 450 nm.
After 60 minutes the AMG development was stopped by rinsing the slices in water and afterwards replacing the developer with a 5% sodium thiosulphate solution for 10 min (the AMG stop bath solution).
In the second stage, imitation dominated and the financing of Y-10 development was stopped.
Although development was stopped for almorexant and for Glaxo's DORA SB-649868, no less than 4 orexin receptor antagonists have reached phase II for insomnia, including Filorexant MK-60966) and Suvorexant MK-43055) froMerckck.
Development was stopped by addition of 1 M H2SO4.
Color development was stopped using 1 N H2SO4.
Color development was stopped using 1 M phosphoric acid.
Colour development was stopped by the addition of 100 µl 0.5 M H2SO4.
HRP substrate was added and color development was stopped with 1N NaOH after 30 minutes incubation.
Color development was stopped by the addition of 100 µl 2 M sulfuric acid.
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