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Color development was performed with hydrogen peroxide/TMB chromogenic substrate, and intensity of the developed color proportionately represented the quantity of NFκB in each sample.
After cooling, development was performed for 6 min at room temperature using MicroChem's SU-8 developer.
Chromatographic development was performed in glass chamber (Macherey Nagel, Germany).
General ELISA processing and procedures for assay development was performed as previously described [26].
Color development was performed with diaminobenzidine (DAB) and counterstaining was done with hematoxylin.
Characterization of intestinal development was performed by histologic and immunohistochemical techniques.
Utility design software development was performed using MATLAB software version 7.8, which is equipped with various advanced applications.
Method development was performed within a temperature interval from 40 °C to 180 °C using water as mobile phase.
Development was performed with diaminobenzidine, using hematoxylin counterstaining.
Color development was performed with DAB substrate (Sigma-Aldrich) and sections were counterstained with hematoxylin.
Colorimetric development was performed with a one-step BCIP-NBT reagent (Sigma).
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