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After development, specimens were treated with 2N HCl for 45 min at room temperature.
After in situ development, specimens were treated with 2 N HCl for 45 min at room temperature, and washed four times with PBSTx (0.3% Triton) for 1 hr.
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As an obvious consequence of ossicular development all specimens of groups S – XL showed advanced hearing abilities compared to specimens of group XS and all specimens of groups S – XL were able to detect frequencies up to 6 kHz.
But this is something new: For eight months, a group of the primitive fish were raised entirely on land so that researchers could compare their development to specimens that grew up in normal, mostly aquatic conditions.
The results showed that the alkaline solution influenced the compressive strength development, and specimens reached more than 60 MPa after 90 days of curing.
For colour development, the specimens were incubated with 3,3′-diaminobenzidine hydrochloride (DAB) supplemented with hydrogen peroxide (DAB Substrat kit, Linaris, Wertheim-Bettingen, Germany) and afterwards counterstained in haematoxylin.
Thus, if exposure occurs during critical periods of a child's development, then specimens should be obtained proximate to those periods.
The aim of this study is the development of specimen geometries able to isolate the plane strain state at a controlled level.
To measure crack development, each specimen is brushed with white cement paint, and 100 × 100 mm grid lines are drawn on it before testing.
For example, the development of specimen holders that are more stable and allow collecting data at slower exposure rates with "resting" periods in between exposures might make biological specimens more impervious to radiation damage (Chen et al. 2008; Karuppasamy et al. 2011).
Three-dimensional finite element (FE) models are developed to study the deformation and damage development in the specimens under dynamic bending.
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