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On day 12 of embryo development, the shell covering the air sac was taped to avoid potentially irritating shell fragments to fall down on the CAM while cutting a hole in the shell with scissors.
Detection of HCMV from clinical specimens was a slow process until the development of shell vial method with staining for immediate early antigen (IEA).
Kurihara et al. observed at very high pCO2 (2268 µatm) that embryonic development and shell formation in Crassostrea gigas was inhibited during the initial 48 h following fertilization [18].
The large variety of genes differentially expressed between developmental stages reveals the regulatory complexity of larval development, including shell formation.
Cold environments, on the other hand, might lead to slow development, smaller shell and smaller clutch sizes, however might result in longer lifespan [ 60].
In marsupials, intimate contact between conceptus and maternal tissues occurs only late in development, after shell breakdown approximately two-thirds of the way through pregnancy [ 47, 48].
The iron-storage Ferritin proteins that in mollusks have been related with immunity, development and shell formation processes [ 32], and LEA (Late Embryogenesis Abundant) proteins that are thought to be involved in the response to drying and osmotic stresses in plants and nematodes [ 33] were also enriched in the whole body.
Taken together, these results suggest multiple functions of the tyrosine metabolic pathway during P. fucata larval development, including shell and periostracum formation.>> -wrap-foot> T/O FC, fold-change (FC) in gene expression comparing trochophore stage larvae with fertilized eggs by Significance Analysis of Microarrays analysis; D/T ratio, U/D ratio, J/U ratio are similar.
During the Cambrian, however, with the development of shells and skeletons, fossilisation was relatively more likely.
Approximately the first 87 percent of Earth history occurred before the evolutionary development of shell-bearing organisms.
Joining the road, I could see plenty of the more recent development: concrete shells of buildings going up and the dusty scars where roads cut across deforested hillsides.
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