Fetal myeloid progenitors derived from PU.1−/− mutant mice can recapitulate macrophage development upon induction of the conditional transgene of PU.1 that is fused with the ligand binding domain of the Oestrogen Receptor (PUER), in response to Tamoxifen (Walsh et al, 2002; Supplementary Figure S1).
The development of appropriate conditional models is crucial to advance our understanding of the molecular mechanisms underlying PTEN-associated carcinogenesis.
As the NMD pathway affects both mRNA isoform distributions as well as transcript levels, unraveling the underlying molecular mechanism(s) for the NMD requirement in proliferation will require full whole-transcriptome mapping studies of NMD deficient mammalian cells as well as the development of a conditional NMD null cell system amenable to rescue experiments.
The development of additional conditional NPY receptor knockout mice in tandem with drug discovery efforts may help this discussion.
Therefore, direct genetic evidence is critical for claims of essentiality, and the development of suitable conditional genetic technologies in Plasmodium that can be scaled up to validate large numbers of drug targets systematically remains a priority.
Hsp90β does not compensate for Hsp90α during germ cell development in the testes of the conditional knockout mice.
We then estimated the univariate odds ratio for the development of pancreatic cancer, conditional on the presence of a first-degree relative affected with pancreatic cancer, based on the two by two table.
The development of conditional transgenic strains in the mouse has particularly assisted in defining our existing understanding of ontological relationships during kidney development.
Moreover, the recent development of conditional somatic mutagenesis in the mouse prostate offers the possibility to generate new models that more faithfully reproduce the human disease, and thus should contribute to improve diagnosis and treatments.
Protein stability can be efficiently controlled by conditional degrons, which induce target protein degradation at restrictive conditions.We used the yeast Saccharomyces cerevisiae for development of a conditional, bidirectional degron to control protein stability, which can be fused to the target protein N-terminally, C-terminally or placed internally.
