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We agree it is important to address the potential non-specific effect of RA experiments, for example on general growth and development of the bud (even in this non-model system which is non-trivial to work with).
Based on the methylation profiles and the morphology observed it was possible to define four phases or stages in the differentiation and development of the bud: a vegetative phase from July to August, which we named phase A (Fig. 1).
In the floral transition of azalea, we observed that, besides the changes in the global DNA methylation and H4 histone acetylation, it was possible to observe a different distribution of 5-mdC AcH4accordingrding to the development of the bud, providing information about what types of cells are the most decisive at each stage of floral development.
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The transition from vegetative to reproductive development of the buds is accompanied by a number of changes in the physiology of the plant.
The growing portion at the apex of the shoot is the terminal bud of the plant, and by the continued development of this bud and its adjacent tissues, the stem increases in height.
This localisation pattern corresponds with the reported AP1 mRNA expression [ 21, 37], where AP1 expression starts in stage 1 flower bud primordia and increases during the development of the flower bud until stage 3.
(ii) is a stage during the ontogenetic development of buds toward bud burst after duration (i) has been completed.
However, in recent studies, the same developmental response function of temperature has been used consistently through the autumn to winter periods [ 15]. (ii) is a stage during the ontogenetic development of buds toward bud burst after duration (i) has been completed.
Together, these results suggest that the appearance of these limb arteries lags the development of the limb bud, and thus may be in response to a changing environment rather than a programmatic or preemptive occurrence.
Stage 44 is morphologically marked by the development of the forelimb bud [ 7, 8].
The homeobox genes Hlx [ 3], Hex [ 4] and the prospero-related homeobox 1 (Prox1) [ 5] are involved in the development of the liver bud.
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