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Among several optical methods based on surface plasmon resonance, enhanced Raman scattering, wave guiding, Bragg diffraction, and photonic bandgaps, the reflective interference spectroscopy [RIfS] method is recognised as particularly promising for the development of label- free biosensing devices [6 10].
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In addition, technical biases can be introduced at the RNA-labeling step, leading to the development of label-free systems in which the fluorophore selectively binds miRNA-probe duplexes [ 16].
The potential for development of label-free, low-density DNA microarrays is demonstrated and is being pursued.
Electrochemical impedance spectroscopy (EIS) can sensitively interpret the resistive ability of an interfacial species, which has been broadly applied in the study of corrosion science as well as development of label-free sensors.
The ligand-responsive ribozyme switches, by transforming the change of TPP concentration into the visual reporter genetic expression in cells, enable efficient development of label-free, noninvasive and high specific biosensors in living mammalian cells.
This work demonstrates that GNPs modified GCE for the immobilization of molecular recognition element is promising platform for the development of label-free electrochemical biosensors for the determination of special proteins.
Optimisation of radiolabelling protocols and the development of labelling modules have simplified radiopharmaceutical preparation, but the desire for further development remains (Ocak et al. 2010).
A novel method for the future development of label-free DNA sensors is proposed here.
Another application potential can be seen in the development of label-free detection systems.
The power of this approach will be greatly enhanced by the development of label-free binding detection compatible with the proteome array format (99).
The development of "label-free" assays addresses many of these limitations, but current approaches still lack the throughput needed for applications in drug discovery.
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