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Therefore, development of a serologic assay was not pursued.
The serologic response to ehrlichial infection may also be blunted or inhibited by tetracycline therapy, which, when given early in the course of Ehrlichia infections, inhibits the development of a serologic response (10, 12 ).
This, in combination with establishing a cell line for the study of KSHV, enabled the development of a serologic test against the latency-associated nuclear antigen (LANA), mirroring an assay previously developed for the EBV homologue EBNA-1.
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Development of a specific serologic assay for ONNV within the SFV antigenic complex would be a valuable tool for diagnosis and surveillance studies.
Development of a standardized serologic test for B. procyonis would allow epidemiologic studies of its prevalence and incidence and help determine factors associated with infection.
Potential cross-reactivity is an important issue to be considered in the development of a new serologic test, particularly for countries with a high incidence of TB, high BCG vaccination coverage and high levels of exposure to environmental mycobacteria [ 35, 36].
These processes are fundamental for the quality of the immune response and for development of an efficient serologic memory to prevent re-infections [1].
The Centers for Disease Control and Prevention CDCC) had, before the attacks, instituted the development of anthrax serologic assays particularly enzyme-linked immunosorbent assays (ELISAs)—for use in anthrax vaccine studies in humans and to provide a standard human reference serum.
To establish if >1 of these newly identified hantaviruses is pathogenic for humans will require development of robust serologic assays (13 ) and application of other sensitive technologies, such as microarray analysis (14, 15 ), for rapid detection of shrewborne hantavirus RNA in human tissues and bodily fluids.
The emergence of sensitive antibody-based diagnostic tests for detection of bovine TB supports the concept of development of a reliable and easy to use serologic test [ 12- 16].
The Cap protein is a major candidate antigen for the development of serologic diagnostic methods as it is the major immunogenic protein containing specific epitopes of PVC2 [ 15].
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