Other approaches in supplying P to pigs include the addition of phytase to the feed, and even the development of phytase transgenic pigs [70] – which, to date, do not appear to have found commercial application.
Nine versions of a phytase gene of bacterial or fungal origin were used to generate transplastomic lines.
Thus, the present study brings out a potentially novel application of phytase for biodegradation of organophosphorus pesticides.
The high substrate specificity of the phytase for sodium phytate indicates that the phytase from B. nealsonii ZJ0702 is highly specific for inositol polyphosphate.
In same broth under optimized conditions for cell growth and phytase production, the transformant reached an OD600 nm value of 1.68 and a phytase yield of 42.12 U/mL, representing approximately 1.6-fold and 3.1-fold increases, respectively, compared to growth in natural milk broth.
For example, a phytase supplemented diet leads to the dephosphorylation of phytase which improves the intestinal uptake of dietary minerals [8, 9].
The treatments consisted of the development of eight diets (reference with and without phytase, oats with or without phytase, rice grits with or without phytase and wheat middlings with or without phytase) in a factorial scheme.
About 350 mg of NF were dipped in a phytase enzyme solution (112.29 unit/mL) and kept in a shaker (100 rpm) for 1 h.
Furthermore, rice bran being a natural agricultural byproduct makes this attempt of stabilization of phytase, a sustainable process.
Fig. 6 Activity of (a) free phytase enzyme and (b) CSNFP, after being exposed to different temperatures.
