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Detailed development methods for the Ct-OATP1B3-C and Ct-OATP1B3-v1 expression plasmids, development of human embryonic kidney (HEK) 293 cells transiently or stably expressing each OATP1B3 isoform, as well as anti-OATP1B3 rabbit serum preparation, are described in Additional file 8 (supplemental materials and methods).
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