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Because head volume is a fixed morphological structure with no change once development has terminated during or shortly after fledging, the difference in head volume between yearlings and older individuals cannot be ascribed to phenotypic plasticity.
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Second, we know from pulse-chase experiments [29] that after six long days, at least 50% of a larval cohort from the southern population has terminated diapause and is irrevocably committed to resumed development.
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By 10 h after dawn on day six, more than 50% of individuals within the southern population have terminated diapause, even though there are no overt signs of development in these larvae [28].
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