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RT-PCR analysis of larvae during early development demonstrated that the β chain transcript is expressed by 19 days post-fertilisation (p.f .. RNA probes specific for the β chain were synthesised and used for in situ hybridisation experiments on 30 day p.f. to 180 day old juvenile larvae.
Quantification of intestinal nuclei at the beginning and end of larval development demonstrated that the extra nuclei of ubc-25 (ok1732 ) arise earlier during embryogenesis.
Moreover, the tobacco proteome analysis from early to late pollen development demonstrated that proteins involved in primary metabolism and starch synthesis, which were required for pollen tube growth [ 31].
Immunoblot analysis of LlBTPC during pollen development demonstrated that LlBTPC accumulation starts after PMI, and the amount increases to the 12 cm flower bud stage, and is sustained at this level until after anthesis.
Injection of antisense morpholinos against islet1 (isl1) and fgf8, genes implicated in mammalian heart development, demonstrated that Isl1 is required for cardiomyocyte differentiation at the venous pole, whereas FGF8 signaling is necessary for cardiomyocyte addition at the arterial pole.
Using the zebrafish as a tool, a set of 16p11.2 homologs was identified, primarily on chromosomes 3 and 12. Use of 11 phenotypic assays, spanning the first 5 days of development, demonstrated that this set of genes is highly active, such that 21 out of the 22 homologs tested showed loss-of-function phenotypes.
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The absence of a phenotype in the Bmp4-deficient telencephalon along with recent genetic studies establishing a role for a BMP4 receptor, BMPRIA, in telencephalic midline development, demonstrate that loss of Bmp4 function in the telencephalon can be compensated for by at least one other Bmp gene, the identity of which has not yet been determined.
A correlation between roughness and the number of attached E. coli cells showed that the higher the roughness at microscale level the higher the biofilm development, demonstrating that, apart from specific interactions at nanoscale surface, heterogeneity at microscale can greatly modify the antibacterial action.
Our results provide the first demonstration that benign breast tissue contains transcriptional alterations that indicate risk of breast cancer development, demonstrating that essential precursor biomarkers of malignancy are present many years prior to cancer development.
We showed that blocking β3-adrenergic receptor using a specific inhibitor virtually completely prevented cold-induced hypercholesterolemia and accelerated plaque development, demonstrating that cold-induced sympathetic activation is crucial for this pathological process.
Knockout mutation in CVY1 caused severe trichome growth defects with relatively mild effects on overall shoot development, demonstrating that CVY1 functions in polarized cell growth and cell shape patterning.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com